Thermococcus camini sp. nov., a hyperthermophilic and piezophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Atlantic Ridge

A coccoid-shaped, strictly anaerobic, hyperthermophilic and piezophilic organoheterotrophic archaeon, strain Iri35c T , was isolated from a hydrothermal chimney rock sample collected at depth of 2300 m the Mid-Atlantic Ridge (Rainbow vent field). Cells grew NaCl concentrations ranging 1–5 % (w/v) (optimum 2.0 %), pH 5.0 to 9.0 7.0–7.5), temperatures between 50 90 °C 75–80 °C) pressures 0.1 least MPa (optimum: 10–30 MPa). The novel isolate on complex organic substrates, such as yeast extract, tryptone, peptone or beef preferentially in presence elemental sulphur l -cystine; however, these molecules were not necessary for growth. Its genomic DNA G+C content 54.63 mol%. genome has been annotated metabolic predictions are accordance with characteristics Thermococcales general. Phylogenetic analyses based 16S rRNA gene sequences concatenated ribosomal protein showed that belongs genus Thermococcus, is closer species T. celericrescens siculi . Average nucleotide identity scores silico DNA–DNA hybridization values genomes type Thermococcus below delineation threshold. Therefore, considering phenotypic data presented, suggested represent species, which name camini sp. nov. proposed, (=UBOCC M-2026 =DSM 111003 ).